Soybean cultivar S06-CL821457

ABSTRACT

The present invention is in the field of soybean variety S06-CL821457 breeding and development. The present invention particularly relates to the soybean variety S06-CL821457 and its progeny, and methods of making S06-CL821457.

THE FIELD OF THE INVENTION

The present invention is in the field of soybean variety S06-CL821457breeding and development. The present invention particularly relates tothe soybean variety S06-CL821457 and its progeny, and methods of making.

BACKGROUND OF THE INVENTION

Soybean Glycine max (L) is an important oil seed crop and a valuablefield crop. However, it began as a wild plant. This plant and a numberof other plants have been developed into valuable agricultural cropsthrough years of breeding and development. The pace of the developmentof soybeans, into an animal foodstuff and as an oil seed hasdramatically increased in the last one hundred years. Planned programsof soybean breeding have increased the growth, yield and environmentalhardiness of the soybean germplasm.

Due to the sexual reproduction traits of the soybean, the plant isbasically self-pollinating. A self-pollinating plant permits pollen fromone flower to be transferred to the same or another flower of the sameplant. Cross-pollination occurs when the flower is pollinated withpollen from a different plant; however, soybean cross-pollination is arare occurrence in nature.

Thus the growth and development of new soybean germplasm requiresintervention by the breeder into the pollination of the soybean. Thebreeders' methods of intervening in the pollination depend on the typeof trait that is being bred. Soybeans are developed for a number ofdifferent types of traits morphological (form and structure),phenotypical, or for traits like growth, day length, temperaturerequirements, initiation date of floral or reproductive development,fatty acid contents, insect resistance, herbicide resistance and yield.The genetic complexity of the trait often drives the selection of thebreeding method.

Due to the number of genes within each chromosome, millions of geneticcombinations exist in the breeders' experimental soybean material. Thisgenetic diversity is so vast that a breeder cannot produce the same twocultivars twice using the exact same starting parental material. Thus,developing a single variety of useful commercial soybean germplasm ishighly unpredictable, and requires intensive research and development.

The development of new soybeans comes through breeding techniques, suchas: recurrent selection, mass selections, backcrossing, single seeddescent and multiple seed procedure. Additionally, markers' assistedbreeding allows more accurate movement of desired alleles or evenspecific genes or sections of chromosomes to be moved within thegermplasm that the breeder is developing. RFLP, RAPD, AFLP, SSR, SNP,SCAR, isozymes, are all forms of markers that can be employed inbreeding soybeans or in moving traits into soybean germplasm. Otherbreeding methods are known and are described in various soybeantextbooks.

When a soybean variety is being employed to develop a new soybeanvariety or an improved variety the selection methods includebackcrossing, pedigree breeding, recurrent selection, modified selectionand mass selection. The efficiency of the breeding procedure along withthe goal of the breeding is the factors for determining which selectiontechniques are employed. A breeder continuous evaluates the success ofthe breeding program and therefore the efficiency of any breedingprocedure. The success is usually measured by yield increase, commercialappeal and environmental adaptability of the developed germplasm.

The development of new soybean cultivars most often requires thedevelopment of hybrid crosses (some exceptions being initial developmentof mutants directly through the use of the mutating agent, certainmaterials introgressed by markers, or transformants made directlythrough transformation methods) and the selection of progeny therefrom.Hybrids can be achieved by manual manipulation of the sexual organs ofthe soybean or by the use of male sterility systems. Breeders often tryto identify true hybrids by a readily identifiable trait or the visualdifferences between Inbred and hybrid material. These heterozygoushybrids are then selected and repeatedly selfed and reselected to formnew homozygous soybean lines.

Mass and recurrent selection can be used to improve populations. Severalparents are intercrossed and plants are selected based on selectedcharacteristics like superiority or excellent progeny. Outcrossing to anumber of different parents creates fairly heterozygous breedingpopulations.

Pedigree breeding is commonly used with two parents that possessfavorable, complementary traits. The parents are crossed to form a F1hybrid. The progeny of the F1 hybrid is selected and the best individualF2s are selected; this selection process is repeated in the F3 and F4generations. The inbreeding is carried forward and at F5-F7 the bestlines are selected and tested in the development stage for potentialusefulness in a selected geographic area.

In backcross breeding a genetic allele or loci is transferred into adesirable homozygous recurrent parent. The trait is in the donor parentand is tracked into the recurrent parent. The resultant plant is likethe recurrent parent with the new desired allele or loci.

The single-seed descent method involves use of a segregating plantpopulation for harvest of one seed per plant. Each seed sample isplanted and the next generation is formed. When the F2 lines areadvanced to F6 each plant will be derived from a different F2. Thepopulation will decline due to failure of some seeds, so not all F2plants will be represented in the progeny.

New varieties must be tested thoroughly to compare their developmentwith commercially available soybeans. This testing usually requires atleast two years and up to six years of comparisons with other commercialsoybeans. Varieties that lack the entire desirable package of traits canbe used as parents in new populations for further selection or aresimply discarded. The breeding and associated testing process is 8 to 12years' of work prior to development of a new variety. Thousands ofvarietal lines are produced but only a few lines are selected in eachstep of the process. Thus the breeding system is like a funnel withnumerous lines and selections in the first few years and fewer and fewerlines in the middle years until one line is selected for the finaldevelopment testing.

The selected line or variety will be evaluated for its growth,development and yield. These traits of a soybean are a result of thevariety's genetic potential interacting with its environment. Allvarieties have a maximum yield potential that is predetermined by itsgenetics. This hypothetical potential for yield is only obtained whenthe environmental conditions are perfect. Since prefect growthconditions do not exist, field experimentation is necessary to providethe environmental influence and to measure its effect on the developmentand yield of the soybean. The breeder attempts to select for goodsoybean yield potential under a number of different environmentalconditions.

Selecting for good soybean yield potential in different environmentalconditions is a process that requires planning based on the analysis ofdata in a number of seasons. Identification of the varieties carrying asuperior combination of traits, which will give consistent yieldpotential, is a complex science. The desirable genotypic traits in thevariety can often be masked by other plant traits, unusual weatherpatterns, diseases, and insect damage. One widely employed method ofidentifying a superior plant with such genotypic traits is to observeits performance relative to commercial and experimental plants inreplicated studies. These types of studies give more certainty to thegenetic potential and usefulness of the plant across a number ofenvironments.

In summary, the goal of the soybean plant breeder is to produce new andunique soybeans and progeny of the soybeans for farmers' commercial cropproduction. To accomplish this the plant breeder painstakingly crossestwo or more varieties or germplasm. Then the results of this cross arerepeatedly selfed or backcrossed to produce new genetic patterns. Neweravenues for producing new and unique genetic alleles into soybeansinclude introducing mutations or transgenes into the genetic material ofthe soybean are now in practice in the breeding industry. These geneticalleles can alter pest resistance such as insect resistance, nematoderesistance, herbicide resistance, or they can alter the plant's diseasetolerance, or its fatty acid compositions, the amount of oil produced,and/or the amino acid compositions of the soybean plant or its seed.

The traits a breeder selects for when developing new soybeans are drivenby the ultimate goal of the end user of the product. Thus if the goal ofthe end user is to resist a certain plant disease so overall more yieldis achieved, then the breeder drives the introduction of genetic allelesand their selection based on disease resistant levels shown by theplant. On the other hand, if the goal is to produce a specific oil, witha high level of oleic acid and a lower level of linoleic acid, then thebreeder may drive the selection of genetic alleles based on levels offatty acids in the seed and accept some lesser yield potentials or otherless desirable agronomic traits.

The new genetic alleles being introduced in to soybeans are widening thepotential uses and markets for the various products and by-products ofthe oil from the seed plants such as soybean. A major product extractedfrom soybeans is the oil in the seed. Soybean oil is employed in anumber of retail products such as cooking oil, baked goods, margarinesand the like. Another useful product is soybean meal, which is acomponent of many foods and animal feedstuffs.

SUMMARY OF THE INVENTION

One embodiment of the invention relates to seed of a soybean cultivardesignated S06-CL821457. The invention relates to the plant from theseed designated S06-CL821457, or the plant parts. The invention alsoencompasses a tissue culture of regenerable cells, cells or protoplastsbeing from a tissue selected from the leaf, pollen, stomatal cell,embryo, meristematic cell, root, root tip, anther, flower, ovule, seed,stem, pod, petal and the cells thereof.

The invention in one aspect covers a soybean plant, or parts thereof,having all of the physiological and morphological characteristics of thesoybean plant.

Another aspect of this invention is the soybean plant seed or derivedprogeny which contains a transgene which provides herbicide resistance,insect resistance, resistance to disease, resistance to nematodes, malesterility, or which alters the oil profiles, the fatty acid profiles,the amino acids profiles or other nutritional qualities of the seed.

The present invention further covers a method for producing a soybeanseed with the steps of crossing at least two parent soybean plants andharvesting the hybrid soybean seed, wherein at least one parent soybeanplant is the present invention. In another aspect of the inventioncovers the hybrid soybean seed and the progeny soybean plant andresultant seed, or parts thereof from the hybrid seed or plant or itsprogeny.

In an additional aspect, the invention covers a method for producing asoybean progeny from the invention by crossing soybean line S06-CL821457with a second soybean plant to yield progeny soybean seed and thengrowing progeny soybean seed.

Yet another aspect of the invention covers a method for a breedingprogram using plant breeding techniques which employ the soybean plantS06-CL821457 as plant breeding material and performing breeding byselection techniques, backcrossing, pedigree breeding, marker enhancedselection, mutation and transformation.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the Geographic Segment Chart—GSEGC shows the breakout forgrain yield at standard moisture for S06-CL821457 across geographiclocations.

FIG. 2 shows the Group Mean chart (GRP_MN=Group Mean) of Grain Yield atstandard moisture for S06-CL821457. This chart shows YieldStability—Win >5% of trial mean, Tie + or −5% of trial mean. Losses <5%of trial mean. The chart's vertical axis=yield of target variety, itshorizontal axis=location average yield. When the target variety line isabove the location average line this is desirable. The RSQ of the targetvariety shows a number. This number when it is closest to 1=yieldstability.

DETAILED DESCRIPTION

The following data is used to describe and enable the present soybeaninvention.

Trait Code Performance Trait Description VHNO Variety/Hybrid NumberYGSMN Grain Yield at Std MST (standard moisture) - YGSMN MRTYN MaturityDays from planting MRTYN HLDGR Harvest Lodging HLDGR PLHTN Plant Height(cm) PRR_R Phytophthora Root Rot To PRR_R IC_(——)R Iron ChlorosisIC_(——)R SDS_R Sudden Death Syndrome SDS_R SCL_R Sclerotinia White MoldSCL_R FELSR Frogeye Leaf Spot FELSR STR_R Shattering STR_R GLDGR GreenLodging GLDGR PLBRR Plant Branching PLBRR EMRGR Emergence EMRGR

Syngenta Soybean Data Collection Traits and Timing Trait Order GroupCode Description Timing Comments # Loc Stage 4 # Loc Stage 5 # Loc Stage6 Scale 1 HS RRG_R Presence of RR V2-V4, 4-7 Home Loc's Home Loc's HomeLoc's Roundup 1 = gene DAYS AFTER R, 5 = seg, SPRAYING 9 = susc, 1 HSRUR_R Roundup Tolerance R1-R2 Home Loc's Home Loc's Home Loc's 1-9scale, 1 = Yellowing no yellowing, 9 is extreme yellowing 1 OTH EMRGREmergence - stand VE-V3 NK EPA_R = 2 4 1 to 9 establishment, V2-V6, 2005(1 = best) uniformity and vigor on use EMRGR 2 DIS IC_(——)R IronDeficiency August Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to 9Chlorosis Nursery (1 = best) 2 DIS ICFLR Iron Deficiency Initial flashV2 Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to 9 Chlorosis YellowJune-July Nursery (1 = best) Flash 2 DIS ICR_R Iron Deficiency 2-3 weeksafter Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to 9 ChlorosisRecovery, ICFLR Nursery (1 = best) (Stunting and/or Green-up) 2 DISPRR_R Phytophthora Scheduled Internal Field St. Joe St. Joe 1 to 9 RootRot Tolerance Nursery (1 = best) in pond for field tolerance 2 NEM SCN_RSCN Field Rating June Internal Field Conesville, Conesville, Field = 1-9Nursery St. Joe St. Joe (1 best) 3 AGR FL_CR Flower Color R1 ConfirmedNumeric for Home Loc's Home Loc's Home Loc's W = White; EDC P = Purple;Seg = Mix 3 AGR PLCNR Plant canopy August Growth OpportunisticOpportunistic 1 to 9, (~R5-R6) expressive 1 = Slender, locations 5 =Inter- mediate, 9 = Bush 3 DIS ARSTR Asian Rust Rating WhenOpportunistic Opportunistic Opportunistic 1 to 9 differences (1 = best)occur 3 DIS BSR_R Brown Stem Rot When Opportunistic OpportunisticOpportunistic 1 to 9 differences (1 = best) occur 3 DIS CR_(——)RCharcoal Rot When Opportunistic Opportunistic Opportunistic 1 to 9differences (1 = best) occur 3 DIS DPC_R Stem Canker When OpportunisticOpportunistic Opportunistic 1 to 9 (Northern) differences (1 = best)occur 3 DIS DPM_R Stem Canker When Opportunistic OpportunisticOpportunistic 1 to 9 (Southern) differences (1 = best) occur 3 DIS FELSRFrogeye Leaf Spot When Opportunistic Opportunistic Opportunistic 1 to 9differences (1 = best) occur 3 DIS SCL_R Sclerotinia When OpportunisticOpportunistic Opportunistic 1 to 9 White Mold differences (1 = best)occur 3 DIS SDS_R Sudden Death When Opportunistic OpportunisticOpportunistic 1 to 9 Syndrome differences (1 = best) occur 3 PERF GLDGRGreen lodging R5 to R6 Where Opportunistic Opportunistic Opportunistic 1to 9 (1 best) differences 1 = All erect; occur 5 = 45 degrees; 9 = flat4 AGR MRTYD Maturity Date R8 Home ~1-2 ~5 ~10 MMDD (MMDD)-95% oflocations + 1-2 (Sept 10 = plants in row have off site/station 0910)mature pod color 4 AGR HC.CR Hilum Color R8 Numeric for Home Loc's HomeLoc's Home Loc's G = Gray, EDC BR = Brown, IB = Imperfect Black; BL =Black; Y = Yellow; BF = Buff; IY = Imperfect Yellow; Seg = Mix 4 AGRPB_CR Pubescence Color R8-Harvest Numeric for Home Loc's Home Loc's HomeLoc's G = Gray; T = EDC Tawny; LT = Lt. Tawny; Seg = Mix 4 AGR PD_CR PodColor R8-Harvest Numberic for Home Loc's Home Loc's Home Loc's T = Tan;B = EDC Brown; Seg = Mix 4 AGR PLBRR Plant branching R8-Harvest GrowthOptional Home Loc's Home Loc's 1 to 9, 1 = no expressive branching; 5 =locations average branching; 9 = profuse branching 4 AGR PLHTN PlantHeight (cm) Harvest Growth Optional Home Loc's Home Loc's Taken in cmexpressive locations. 4 DIS GS_(——)R Green Stem Harvest WhereOpportunistic Opportunistic Opportunistic 1 to 9 differences (1 = best)occur 4 PERF HLDGR Harvest Lodging R8-Harvest Where OpportunisticOpportunistic Opportunistic 1 to 9 (1 best) differences 1 = All erect;occur 5 = 45 degrees; 9 = flat 4 PERF HVAPR Harvest Appearance HarvestWhere Opportunistic Opportunistic Opportunistic 1 to 9, differences 1 =Excellent, occur 5 = Avg, 9 = Poor 4 PERF STR_R Shattering Harvest Hillplot Opportunistic + Opportunistic + 1 to 9 planter, 2006? Bay and St.Joe Bay and St. Joe (1 = best) Order 1 = Seedling, 2 = Vegetative, 3 =Reproductive to grain-fill, 4 = Maturity Opportunistic ratings shouldonly be collected if differences occur. At least one variety has arating of 5 or greater and there is a spread of 3 in the ratings.Roundup is a trademark of Monsanto Trait Definitions OpportunisitcRatings developed in YT. Opportunistic ratings should only be collectedif differences occur. At least one variety has a rating of 5 or greaterand there is a spread of 3 in the ratings. (Scale 1-9, 1 = Best).Emergence (EMRGR) A rating of the uniform establishment and growth ofseedlings. Taken from V1-V3, (Scale 1-9). Maturity (MRTYD) The month andday (MMDD) when 95% of the main stem pods in the plot have reached theirmature color. Plant Height (PLHTN) The average measured plant height incm. Branching (PLBRR) Rating of the number of branches and theirrelative importance to yield. Taken at growth expressive locations.(Scale: 1-9, 1 = stick, no branching, 3 = 1 seed bearing branch, 5 =average branching or 2-3 seed bearing branches, 7 = 3-4 seed bearingbranches, 9 = profuse branching). Green Lodging (GLDGR) Rating based onthe average of plants leaning from vertical in R5 to R6 stage (Scale1-9, 1 to 9 (1 best) 1 = All erect; 5 = 45 degrees; 9 = flat). HarvestLodging (HLDGR) Rating based on the average of plants leaning fromvertical at harvest (scale 1-9, 1 to 9 (1 best) 1 = All erect; 5 = 45degrees; 9 = flat). Shatter (STR_R) Rating of pre-harvest loses based onamount of plants with open pods (Scale 1-9). Iron Deficiency Chlorosis(IC_(——)R) final rating = average of initial Yellow Flash (ICFLR) andrecovery (ICR_R) taken 2-3 weeks after initial yellow flash. (Scale 1-9,1 = Best). LS means analysis is unequal entries and reps between years.Soybean Cyst Nematode (CN_1R, CN_3R, CN_5R, CN14R) Greenhouse screen-30day screen using infested soil. Rating Scale based upon femalereproduction index on a susceptible check set where <10% = R; <30% = MR;<60% = MS; >60% = S. In priority order, the races screened include; 3,14, 1 & 5. Phytophthora Root Rot Field tolerance (PRR_R) or actual gene(RPS_T). Sudden Death Syndrome (SDS_R) based on leaf area affected,scale 1-9. Can be GH or field. Brown Stem Rot (BSR_R) Greenhousepot-root dip or field rating of leaf symptoms. (Scale 1-9) Root KnotNematode Arenaria (MA_(——)R), Incognita (MI_(——)R), Javanica (MJ_(——)R)RKN). Scale 1-9. Stem Canker North (DPM_R) Southern (DPM_R). Scale 1-9.Sulfentrazone (SUL_R) Greenhouse nursery rating damage of multiplerates. Scale 1-9. Metributzin (MET_R) Greenhouse nursery rating damageof multiple rates. Scale 1-9. Hypocotyl Elongation (HYP_R) A rating of avariety's hypocotyl extension after germination when planted at a 5″depth in sand and maintained a warm germination environment for 10 days.(Scale 1 = Long, 5 = Intermediate, 9 = Short)

TRAIT DEFINITIONS

Hypocotyl Elongation (HYPO) A rating of a variety's hypocotyl extensionafter germination when planted at a 5″ depth in sand and maintained awarm germination environment for 10 days.

Seedling Establishment (EMG) A rating of the uniform establishment andgrowth of seedlings.

Maturity (MAT) The number of days after Aug. 31 when 95% of the mainstem pods in the plot have reached their mature color.

Peroxidase Activity (Perox)—seed protein peroxidase activity is definedas a chemical taxonomic technique to separate cultivars based on thepresence or absence of the peroxidase enzyme in the seed coat. Ratingsare POS=positive for peroxidase enzyme or NEG=negative for peroxidaseenzyme.

Plant Height (PLTHT) The average measured plant height in centimeters.

Branching (BRANCH or PLBRR) Rating of the number of branches and theirrelative importance to yield. This rating is taken at growth expressivelocations.

Green Lodging (GLODGE or GLDGR) Rating based on the average of plantsleaning from vertical in R5 to R6 stage.

Stem Lodging (LODGE) Rating based on the average of plants leaning fromvertical at harvest. Lodging score (1=completely upright, 9=completelyprostrate),

Shatter (SHAT or STR_R) Rating of pre-harvest loses based on amount ofplants with open pods.

Iron Deficiency Chlorosis (IDC or IC_R) A composite rating of YellowFlash, Green-up, and Stunting in HpH soil. A 1 rating is the mostpositive for resistance to the symptoms listed and 9 is the leastpositive.

Phytophthora Root Rot (PGR) or (PFT) Greenhouse pot—root dip method forPFT and hypodermic needle method for rating PGR.

Root Knot Nematode (RKN) Greenhouse screen—30 day screen using infestedsoil. Rating Scale based upon female reproduction index on a susceptiblecheck set where <10%=R; <30%=MR; <60%=MS; >60%=S.

Stem Canker (STC) Based on number of lesions, scale 1-5.

Sulfentrazone (SULF) Authority™ (commercial herbicide) Greenhousenursery rating damage of multiple rates.

Metributzin (MET) Greenhouse nursery rating damage of multiple rates.

DEFINITIONS OF STAGING OF DEVELOPMENT

The plant development staging system employed in the testing of thisinvention divides stages as vegetative (V) and reproductive (R). Thissystem accurately identifies the stages of any soybean plant. However,all plants in a given field will not be in the stage at the same time.Therefore, each specific V or R stage is defined as existing when 50% ormore of the plants in the field are in or beyond that stage.

The first two stages of V are designated a VE (emergence) and VC(cotyledon stage). Subdivisions of the V stages are then designatednumerically as V1, V2, V3 through V (n). The last V stage is designatedas V (n), where (n) represents the number for the last node stage of thespecific variety. The (n) will vary with variety and environment. Theeight subdivisions of the reproductive stages (R) states are alsodesignated numerically. R1=beginning bloom; R2=full bloom; R3=beginningpod; R4=full pod; R5=beginning seed; R6=full seed; R7=beginningmaturity; R8=full maturity.

BROWN STEM ROT (BSR)—This disease is caused by the fungus Phialophoragregata. The disease is a late-season, cool-temperature, soilbornefungus which in appropriate favorable weather can cause up to 30 percentyield losses in soybean fields. For purposes of these tests theinformation is gathered in a greenhouse with a plant in a pot then aroot dip procedure is employed.

SUDDEN DEATH SYNDROME (SDS or SDS_R)—This disease is caused byslow-growing strains of Fursarium solani that produce bluish pigments inculture. The disease is a mid to late season, soil borne disease thatoccurs in soybean fields with high yield potential. Yield losses may betotal or severe in infected fields. Sudden Death Syndrome (SDS) is basedon leaf area affected. The scale used for these tests is 1-5 or ifidentified as SDS_R the scale is 1-9.

SOYBEAN CYST NEMATODE—The Soybean Cyst Nematode (SCN) Heteroderaglycines, is a small plant-parasitic roundworm that attacks the roots ofsoybeans. Soybean Cyst Nematode (SCN) for purposes of these tests isdone as a greenhouse screen—30 day screen using infested soil. Therating scale is based upon female reproduction index on a susceptiblecheck set where <10%=R (RESISTANT); <30%=MR (MODERATELY RESISTANT);<60%=MS (MODERATELY SUSPECTIBLE); >60%=S (SUSPECTIBLE). In priorityorder, the screening races include: 3, 14, & 1.

MATURITY DATE. Plants are considered mature when 95% of the pods havereached their mature color. The number of days is either calculated fromAugust 31 or from the planting date. (MR#) wherein # equals days.

RELATIVE MATURITY GROUP (RM). Industry Standard for varieties groups,based day length or latitude. Long day length (northern areas in theNorthern Hemisphere) is classified as (Groups 000, 00, 0,). Mid daylengths variety groups lie in the middle (Groups I-VI). Very short daylengths variety groups (southern areas in Northern Hemisphere) areclassified as (Groups VII, VIII, IX).

SEED YIELD (Bushels/Acre). The yield in bushels/acre is the actual yieldof the grain at harvest.

SHATTERING. The rate of pod dehiscence prior to harvest. Pod dehiscenceinvolves beans dropping out of the pods. Shatter (SHAT) for these teststhe rating of pre-harvest loses is based on amount of plants with openpods.

PLANT. Means the plant, the plant's cells, plant protoplasts, plantcells of tissue culture from which soybean plants can be regenerated,plant calli, plant clumps, and plant cells that are intact in plants orparts of the plants, such as pollen, nodes, roots, flowers, seeds, pods,leaves, stems, pod and the like.

The present invention is S06-CL821457 a mid Group 0 maturity soybean.This soybean is developed for use of the beans. S06-CL821457 is adaptedand researched and developed for upper Midwest where other mid Group 0maturity soybeans are grown. Specific area where best adaptation occursincludes: Widely adapted in maturity zone. This soybean variety carriesthe Phytophthora Root Rot resistance in the form of the Rsp 1c gene.

This soybean variety in one embodiment carries one or more transgenes,for example, the glyphosate tolerance transgene, a desaturase gene orother transgenes. In another embodiment of the invention does not carryany herbicide resistance traits. In yet another embodiment of theinvention, the soybean does not carry any transgenes but carries allelesfor aphid resistance, cyst nematode resistance and/or brown stem or thelike.

The traits of the invention are listed below.

Traits

Plant Characteristics RR ® Y STS ® N Flower Color P Pubescence Color TPod Color B Hilum Color IY % Protein 13% mst. 35.8 % Oil @ 13% mst. 18.8Seed Size/Lb 2838.0 Stem Termination 2.0

Plant Health Rps Gene 1C Phytopthora Root Rot Tolerance 3.7 SCN RACE 1Fl % SCN RACE 3 Fl % SCN RACE 5 Fl % SCN RACE 14 Fl % Root KnotNermatode - Incognita Root Knot Nermatode - Arenaria Stem Canker(Southern) Stem Canker Tolerance (Southern) Rps gene indicates thespecific gene for resistance but if none are indicated then none areknown to be present Y = Yes, has trait. N = no does not contain traitSCN = Soybean cyst nematode RoundUp Ready ®, Roundup ® and Roundup ®Ultra are trademarks of Monsanto Company. STS ® is a trademark ofDuPont. Ratings are on a 1 to 9 scale with 1 being the best.

The instant invention provides methods and composition relating toplants, seeds and derivatives of the soybean cultivar S06-CL821457.Soybean cultivar S06-CL821457 has superior characteristics. TheS06-CL821457 line has been selfed sufficient number of generations toprovide a stable and uniform plant variety.

Cultivar S06-CL821457 shows no variants other than expected due toenvironment or that normally would occur for almost any characteristicduring the course of repeated sexual reproduction. Some of the criteriaoften used to select in various generations include: seed yield,emergence, appearance, disease tolerance, maturity, plant height, andshattering data.

The inventor believes that S06-CL821457 is similar to the comparisonvarieties shown in the last table. However, as shown in the table,S06-CL821457 differs from these cultivars.

Direct comparisons were made between S06-CL821457 and the listedcommercial varieties. Traits measured included yield, maturity,moisture, lodging, plant height, branching, field emergence, protein andoil. The results of the comparison are presented in below. The number oftests in which the varieties were compared is shown.

The present invention S06-CL821457 can carry genetic engineeredrecombinant genetic material to give improved traits or qualities to thesoybean. For example, but not limited to, the present invention cancarry the glyphosate resistance gene for herbicide resistance as taughtin the Monsanto patents (WO92/00377, WO92/04449, U.S. Pat. No. 5,188,642and U.S. Pat. No. 5,312,910) or STS mutation for herbicide resistance.Additional traits carried in transgenes or mutation can be transferredinto the present invention. Some of these genes include genes that givedisease resistance to sclerotinia such as the oxalate oxidase (Ox Ox)gene as taught in PCT/FR92/00195 Rhone Polunc and/or an oxalatedecarboxylase gene for disease resistance or genes designed to alter thesoybean oil within the seed such as desaturase, thioesterase genes(shown in EP0472722, U.S. Pat. No. 5,344,771) or genes designed to alterthe soybean's amino acid characteristics. This line can be crossed withanother soybean line which carries a gene that acts to provide herbicideresistance or alter the saturated and/or unsaturated fatty acid contentof the oil within the seed, or the amino acid profile of the seed.

The present invention S06-CL821457 is employed in a number of plotrepetitions to establish trait characteristics. The present invention isgrown in a number of regions for testing and researching the inventionin various environmental interactions.

GEOGRAPHIC SUMMARY

The target variety yield is given as a percent of the trial average atall locations shown in FIG. 1 and each geographic segment West to Eastor Central (Cntrl) to South East where there are three or morelocations. The plots for these trials are 17.5 plots with 30-inch rows.The plants in the plots are a combination of experimental material andcommercial material. There are usually 36 varieties and there areapproximately 300 plants of each variety with two replications in 20-25locations. The data in FIG. 1 is only charted if there are at least 3 ormore locations. N>=3

The present invention differs from the comparison commercial soybeanlines in that it is exceeding the mean of the group of soybeans (GRP_MN)that were tested and displayed in this geographic segment chart (GSEGC).When all of the regions' data are combined the present invention isabout 103.81% the group mean. Additionally, this invention works verywell in the West where it is above the Group Mean at 105.73%. The datain the Central region may be a bit disappointing compared to the West.In the Central region the present invention is at 98.93% of the yield ofthe Group Mean.

The present invention S06-CL821457 (identified herein as CL821457) isemployed in a trial for testing a number of characteristics. The resultsof the grain yield at standard moisture are shown in the chart shown inFIG. 2. The present invention is a variety that will out yield mostsoybeans across all of the yielding environments. If the environment isextremely low yielding the present invention also tends to out performwhen compared to the group mean. These tests allow the usefulness of theinvention to be shown in light of the environmental geneticinteractions.

Research Data 2004-2005 Yield Performance Chart VHNO YGSMN MRTYN HLDGRPLHTN PRR_R IC_(——)R SDS_R SCL_R FELSR STR_R GLDGR PLBRR EMRGR CL82145749.2 123.0 3.1 77.2 3.7 4.5 3.5 4.9 4.0 3.0 Cultivar 1 48.5 120.3 2.473.0 3.4 5.0 2.9 3.1 2.8 3.6 Cultivar 2 46.9 119.3 4.1 74.6 3.2 3.6 4.84.9 4.7 3.8 Cultivar 3 46.1 122.7 2.2 77.7 4.7 5.7 4.7 4.3 2.0 3.6Environ- 19.0 6.0 6.0 1.0 2.0 7.0 4.0 4.0 2.0 3.0 ments Mean 47.3 120.93.3 75.2 3.7 4.8 4.1 4.7 3.8 3.2 LSD (0.05) 1.6 0.9 1.3 1.3

Each of these lines has their own positive traits. Each of these linesis different from the present invention. The present invention hashigher yield at standard moisture than do the comparison lines. Thepresent invention shows a better rating for emergence than do any of theother lines. The ID-R rating is slightly lower (1=best) than any of thecommercial lines except one. Thus, indicating stronger IDC resistancethan is shown by the other lines. The yield and other data is a snapshotof each of these lines' results in the specific environment and willdiffer when other environmental interactions are measured.

This invention also is directed to methods for producing a new soybeanplant by crossing a first parent plant with a second parent plantwherein the first or second parent plant is the present invention.Additionally, the present invention maybe used in the varietydevelopment process to derive progeny in a breeding population orcrossing. Further, both first and second parent plants can come from thesoybean line S06-CL821457. A variety of breeding methods can be selecteddepending on the mode of reproduction, the trait, and the condition ofthe germplasm. Thus, any such methods using the S06-CL821457 are part ofthis invention: selfing, backcrosses, recurrent selection, massselection and the like.

The scope of the present invention includes any use on S06-CL821457 ofmarker methods. Through the use of markers such as SSRs, RFLP's, SNPs,Ests, AFLPs, gene primers, and the like to identify genetic alleleswhich can be identified and breed with marker assistance into thepresent invention with little to no superfluous germplasm being draggedinto the present invention. This results in formation of the presentinvention plus for example, cyst nematode resistance, brown stem rotresistance, aphid resistance, phytothora resistance, IDC resistance, BTgenes or male sterility genes or glyphosate tolerance genes or droughttolerance genes or alleles and the like. Transgenes maybe directlyintroduced into cultivar using genetic engineering and transformationtechniques well known in the art, some of which are described above, orare originally introduced into a donor, parent using genetic engineeringand transformation techniques, and using the donor in a marker assistedtrait conversion process, the trait is moved for example bybackcrossing. A transgene typically comprises a nucleotide sequencewhose expression is responsible or contributes to the trait, under thecontrol of a promoter capable of directing the expression of thenucleotide sequence at the desired time in the desired tissue or part ofthe plant. Constitutive, tissue-specific or inducible promoters all havedifferent purposes and each could be employed. The transgene may alsocomprise other regulatory elements such as for example translationenhancers or termination signals. The transgene may be adapted to betranscribed and translated into a protein, or to encode RNA in a senseor antisense orientation such that it is not translated or onlypartially translated.

The scope of the present invention also includes any use on S06-CL821457of transformation methods. Transformation methods are means forintegrating new genetic coding sequences (transgenes) into the plant'sgenome by the incorporation of these sequences into a plant throughman's assistance. Many dicots including soybeans can easily betransformed with Agrobacterium. Methods of introducing desiredrecombinant DNA molecule into plant tissue include the direct infectionor co-cultivation of plant cells with Agrobacterium tumefaciens, Horschet al., Science, 227:1229 (1985). Descriptions of Agrobacterium vectorsystems and methods are shown in Gruber, et al., “Vectors for PlantTransformation, in Methods in Plant Molecular Biology & Biotechnology”in Glich et al., (Eds. pp. 89-119, CRC Press, 1993). Transformed plantsobtained via protoplast transformation are also intended to be withinthe scope of this invention. The most common method of transformationafter the use of agrobacterium is referred to as gunning ormicroprojectile bombardment. This process has small gold-coatedparticles coated with DNA (including the transgene) shot into thetransformable material. Techniques for gunning DNA into cells, tissue,explants, meristems, callus, embryos, and the like are well known in theprior art. The DNA used for transformation of these plants clearly maybe circular, linear, and double or single stranded. Usually, the DNA isin the form of a plasmid. The plasmid usually contains regulatory and/ortargeting sequences which assists the expression of the gene in theplant. The methods of forming plasmids for transformation are known inthe art. Plasmid components can include such items as: leader sequences,transit polypeptides, promoters, terminators, genes, introns, markergenes, etc. The structures of the gene orientations can be sense,antisense, partial antisense, or partial sense: multiple gene copies canbe used.

After the transformation of the plant material is complete, the nextstep is identifying the cells or material, which has been transformed.In some cases, a screenable marker is employed such as thebeta-glucuronidase gene of the uidA locus of E. coli. Then, thetransformed cells expressing the colored protein are selected for eitherregeneration or further use. In many cases, a selectable markeridentifies the transformed material. The putatively transformed materialis exposed to a toxic agent at varying concentrations. The cells nottransformed with the selectable marker, which provides resistance tothis toxic agent, die. Cells or tissues containing the resistantselectable marker generally proliferate. It has been noted that althoughselectable markers protect the cells from some of the toxic affects ofthe herbicide or antibiotic, the cells may still be slightly affected bythe toxic agent by having slower growth rates. If the transformedmaterial was cell lines then these lines are regenerated into plants.The cells' lines are treated to induce tissue differentiation. Methodsof regeneration of cellular are well known in the art. The plants fromthe transformation process or the plants resulting from a cross using atransformed line or the progeny of such plants are transgenic plantsthat carry the transgene.

DEPOSIT INFORMATION

Applicants have made a deposit of at least 2500 seeds of soybeancultivar S06-CL821457 with the American Type Culture Collection (ATCC),Manassas, Va. 20110 on Feb. 5, 2008 and designated PTA-8912. The seedswere tested on Feb. 29, 2008 and found to be viable. Access to thisdeposit will be available during the pendency of the application to theCommissioner for Patents and persons determined by the Commissioner tobe entitled thereto upon request. Upon granting of a patent on anyclaims in the application, the Applicants will make the depositavailable to the public pursuant to 37 CFR §1.808. Additionally,Applicants will meet the requirements of 37 CFR §1.801-1.809, includingproviding an indication of the viability of the sample when the depositis made. The ATCC deposit will be maintained in that depository, whichis a public depository, for a period of 30 years, or 5 years after thelast request, or for the enforceable life of the patent, whichever islonger, and will be replaced if it becomes nonviable during that period.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

1. A soybean seed designated S06-CL821457, a sample of said seeddeposited under ATCC Accession No. PTA-8912.
 2. A plant, or partsthereof, produced by growing the seed of claim
 1. 3. Pollen of the plantof claim
 2. 4. A soybean plant, or parts thereof, having all of thephysiological and morphological characteristics of the soybean plant ofclaim
 2. 5. A tissue culture of regenerable cells of the soybean plantof claim
 2. 6. The tissue culture according to claim 5, wherein thecells are obtained from the group consisting of leaf, pollen, embryo,meristematic cell, root, root tip, anther, stomatal cell, flower, seed,stem and pod.
 7. A soybean plant regenerated from the tissue culture ofclaim 6, having all of the morphological and physiologicalcharacteristics of soybean cultivar S06-CL821457.
 8. A method forproducing a soybean seed comprising crossing two soybean plants andharvesting the resultant soybean seed, wherein at least one soybeanplant is the soybean plant of claim
 2. 9. A method for producing ahybrid soybean seed comprising crossing the soybean plant according toclaim 2 with a second soybean plant and harvesting the resultant hybridsoybean seed.
 10. A method for producing a S06-CL821457-derived soybeanplant, comprising: a) crossing soybean line S06-CL821457, a sample ofsaid line deposited under ATCC Accession No. PTA-8912, with a secondsoybean plant to yield progeny soybean seed; and b) growing said progenysoybean seed to yield said S06-CL821457-derived soybean plant.
 11. Themethod of claim 8, wherein the second soybean plant is transgenic. 12.The method of claim 11 wherein the transgenic soybean plant containsgenetic material conferring a trait selected from the group consistingof herbicide resistance, nematode resistance, insect resistance,resistance to disease, and male sterility.
 13. The method of claim 12wherein the resistance to disease is through an oxalate oxidase encodingpolynucleotide sequence or an oxalate decarboxylase encodingpolynucleotide sequence.